In collaboration with our expert Cardiologists, NovoPath performed histological analysis of novel polymeric materials under investigation for use in synthetic heart valves, for evidence of thrombus formation following exposure to trial participant blood in Cardiology.
As a non-standard material for histological testing with properties unlike tissues, investigations were necessary to determine the best method for sample preparation prior to staining including the choice of fixative and whether processing to paraffin was necessary.
Staining of the samples was performed using a modified Masson Trichrome stain, to identify platelets, erythrocytes, fibrin and other connective tissues that may have adhered to the polymer during exposure to blood flow.
NovoPath’s work aided in the determination of the biocompatibility of the polymer for use in the manufacture of synthetic prosthetic heart valves. Following publication, the study is moving to human trials of the valves.